Gene set enrichment analysis (GSEA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the TCGA database showed that genes composing the actin cytoskeleton and focal adhesion program signatures, two programs associated with metastatic properties of cancer cells, were highly enriched in ovarian tumor samples with high RSPO2 expression (Figure 3A). This evidence concerns the gene RSPO2 and cancer.