To further screen target genes, the Venn analysis of these predicted target genes with downregulated differentially expressed genes in Tsc2 − / − MEFs as compared with Tsc2 + / + MEFs and decreased differentially expressed genes in angiofibroma (AF) of TSC patients vs. normal skin obtained only one candidate, MBNL1 [32, 33] (Fig. 4B, Supplementary Tables S8 and S9). The gene discussed is MBNL1; the disease is tuberous sclerosis.