Moreover, we also detected a significant increase in the number of CD4+ CD69+ T cells in the brain tissue of APP/PS1 mice (APP/PS1 + PBMT: 0.26%; APP/PS1: 0.12%) and 3xTg-AD mice (3xTg-AD + PBMT: 0.31%; 3xTg-AD: 0.19%) after PBMT treatment (Fig. 4F; Additional file 1: Fig. S3A). This evidence concerns the gene APP and Alzheimer disease.