Subsequently, in order to explore whether the increase of IFN-γ/IL-10 concentration in serum and brain tissue caused by PBMT treatment of lymph nodes from APP/PS1 and 3xTg-AD mice were related to CD4+ T cells, we performed flow cytometry to detect the number of CD4+ IFN-γ+ T cells and CD4+ IL-10+ T cells in brain and spleen of each group. Here, CD4 is linked to Alzheimer disease.