More importantly, by co-cultivating the PBMT-treated conditioned medium of T lymphocytes from APP/PS1 and 3xTg-AD with the corresponding NSCs derived from APP/PS1 and 3xTg-AD, we found that the co-culture of PBMT-treated T lymphocyte conditioned medium with NSCs promoted the differentiation of NSCs and upregulated the expression of Tuj1 and PSD95. This evidence concerns the gene DLG4 and Alzheimer disease.