In the present study, our results showed that omega-3, especially DHA, remarkably suppressed the migration and invasion capability of lung cancer cells together with a decreased CCL18 expression in NSCLC A549 and 95D cells, indicating that omega-3 (especially DHA) effectively suppress the migration and invasion of NSCLC cells possibly by downregulating CCL18 expression. This evidence concerns the gene CCL18 and lung cancer.