While CD4-Cre Rab27afl/fl mice displayed no difference compared to controls during DSS colitis or at baseline (Supplemental Figure 3C), CD11c-Cre Rab27afl/fl animals (CD11c-Rab27A cKO) exhibited greater weight loss and colonic shortening following DSS treatment, indicating that myeloid cells, including DCs and some macrophages, within the hematopoietic compartment utilize RAB27A to restrict DSS colitis phenotypes (Figure 3, A–D). This evidence concerns the gene CD4 and colitis.