Previous in vitro literature indicates an immune-regulatory role for DC- and T cell–derived EVs, and our observed alterations of the myeloid and T cell compartments in Vav-Rab27a cKO mice during intestinal inflammation prompted us to generate more specific cKO animals to identify relevant immune cell types involved in worsened colitis in the absence of hematopoietic Rab27a. This evidence concerns the gene RAB27A and inflammatory response.