We evaluated the levels of p‐p65 and p‐IκBκB in HCC cells via Western blotting and found that HULC overexpression was associated with a significant increase in p‐p65 and p‐IκBκB levels, whereas the opposite was observed following HULC knockdown (Figure 5A,B). This evidence concerns the gene IKBKB and hepatocellular carcinoma.