Interestingly, upstream regulator analysis identified TNF (p = 2.34 × 10-36), TGFB1(p = 9.02 × 10-36) and IL-1B (p = 2.39 × 10-32) as known senescence and arthritis associated regulators of DEG miRNAs (Figure 2G). This evidence concerns the gene TNF and arthritic joint disease.