We examined the accumulation of trapped TOP2 using the ICE bioassay (Anand et al., 2018; Nitiss et al., 2021)by transiently transfecting RH30 cells, a pediatric rhabdomyosarcoma cell line with siRNA against MRE11 or non-targeting siRNA (Figure 1A) followed by treatment with etoposide at various concentrations (2, 10, 50 μM) for 2 h. The gene discussed is MRE11; the disease is rhabdomyosarcoma.