We simultaneously examined NEK2 protein levels in ccRCC cell lines, namely, 786-O, Caki-1, A-498, and HK-2 cells, and the results suggested that NEK2 was more highly expressed in ccRCC cell lines than in HK-2 cells and that NEK2 expression was effectively increased in ccRCC cell lines, which also further corroborated previous examinations at the cellular and tissue levels. The gene discussed is NEK2; the disease is nonpapillary renal cell carcinoma.