The mRNA expression of TRX1, GCLC, and NQO1 decreased (Figure 2F), the protein expression of Keap1 increased, and Nrf2, NQO1, and HO-1 decreased in the CON + FMT (HFD) group (Figure 2G), indicating that NAFLD induced by HFD closely related to the gut microbiome, and the oxidative liver injury mediated by the Nrf2 pathway played an important role in it. This evidence concerns the gene GCLC and metabolic dysfunction-associated steatotic liver disease.