The following biomarker combination was identified for the detection of BC in males: urine NSE, serum PAI-1/tPA, urine midkine, urine NGAL, urine MMP-9/TIMP-1 and serum prolactin: AUROC 0.795 (95%CI 0.751-0.839) (sensitivity 71.8%, specificity 72.8%). The gene discussed is TIMP1; the disease is breast cancer.