ssGSEA analysis showed that the CHRM2 gene was negatively associated with cellular response to hypoxia, tumor proliferation signature, DNA repair, G2M checkpoint, MYC targets, IL-10 anti-inflammatory signaling pathway, DNA replication, and positively associated with EMT markers, ECM-related genes, angiogenesis, TGFB, collagen formation (Figure 7). The gene discussed is CHRM2; the disease is neoplasm.