RT-qPCR results showed that TIM-3 mRNA in four cases who harbored TIM-3 mutations, including familial 1’s proband and her familial members, was significantly lower than that of three sporadic T-cell lymphoma cases or three healthy controls (Figure 3); In the corresponding HEK293 cells loading TIM-3 mutant, the TIM-3 mRNA expression was also significantly downregulated, suggesting the Tyr82Cys and Arg89Cys mutation in TIM-3 gene is a loss-of -function mutation. This evidence concerns the gene HAVCR2 and T-cell non-Hodgkin lymphoma.