Western blot analyses revealed that the protein abundance of MOF with histone H4K16 acetylation exhibited a remarkably attenuated expression in ERα+ BC cells (MCF7 and T47D) compared with that in ERα− BC cells (MDA-MB-231 and HCC1937), and histone H4K16 acetylation also showed a similar pattern with MOF expression (Figure 1C). This evidence concerns the gene KAT8 and breast cancer.