To identify the novel transcripts originated from SCR during NA+ Rβ infection, virus constructs of NA alone or NA with wild type Rβ, Rβ1091-1190 (deleted for a part of SCR), and Rβ187-55 (deleted for a sequence region downstream to βC1 ORF) were inoculated in N. benthamiana plants and total RNA isolated at 19 dpi was subjected to northern blotting using an SCR specific radiolabeled probe. This evidence concerns the gene RB1 and infection.