In the present study, we evaluated the G6PD gene variant distribution in Wuhan District by screening 430,806 neonates born from November 2016 to December 2021, and the G6PD deficiency rate was 0.22% (958/430,806), with an increase in the prevalence of G6PD deficiency in Wuhan birth cohort relative to the prevalence (0.18%) in our previous study (Shen et al., 2019). Here, G6PD is linked to G6PD deficiency.