To test whether the infection of Mycobacteria could induce ferroptosis in vivo, 8-week ICR mice were infected with the Mtb-attenuated strain BCG at a dose of 5 × 106 CFU/mouse in 100 μl volume via tail vein injection, and the ferroptosis was assessed by the expression of Gpx4 and HO-1 proteins in lungs at 30 days post-infection (Figure 2A). This evidence concerns the gene GPX4 and infection.