Dual expression of PD-1 and LAG3 on T cells has traditionally been considered a marker of a hyper-exhausted phenotype; however, emerging evidence indicates these cells are, in fact, tumor-specific, have increased cytotoxicity and proliferative capacity, and are more activated.46 We observed both increased expression levels of LAG3 (Figure 4f), PD-1 (Figure 4g) and an increased frequency of PD-1+LAG3+CD8+ T cells (Figure 4e) in Responders in comparison to other treatment groups. This evidence concerns the gene CD8A and neoplasm.