To identify the roles of PRRT3-AS1 in the development and progression of SKCM cells, we set up a PRRT3-AS1 downregulation model by siRNA transfection, after which we performed qPCR, wound healing, and transwell assays to explore the roles of PRRT3-AS1 in tumor migration in A2058 and SK-MEL-28 cell lines. The gene discussed is PRRT3; the disease is neoplasm.