ACSL4 and brain ischemia: In vitro model of cerebral ischemia, increases in acyl-coa synthetase long-chain family member 4 (ACSL4) and 12/15-lipoxygenase (12/15-LOX) have been shown to increase polyunsaturated fatty acids (PUFAs) such as arachidonic acid (AA) and adrenal acid (AdA).