DUSP22 and hepatocellular carcinoma: Additionally, in vitro results using HepG2 and SMMC-7721 cells transfected with Ad-shDUSP22 or Ad-DUSP22 showed that DUSP22 knockdown significantly facilitated the cell proliferation, while its overexpression markedly suppressed HCC cell growth compared with corresponding control groups by CCK-8 and EdU staining (Supplementary Fig. 24e–i).