Given the close correlation between FAK blockage with the activity of PTP-motif domain containing C88S in DUSP22, an AAV8-TBG-loaded WT full-length DUSP22 sequence vector (AAV-TBG-DUSP22) and AAV8-TBG-loaded DUSP22 with PTP-motif domain deletion vector (AAV-TBG-DUSP22 (ΔPTP motif)) were produced and introduced to further examine the effects of DUSP22 with PTP-motif mutants on HFHC-caused NASH pathologies in vivo (Supplementary Fig. 20a). The gene discussed is PTK2; the disease is metabolic dysfunction-associated steatohepatitis.