Furthermore, HTqPCR assay showed that NASH serum-caused expression changes of a total of 128 genes mediating inflammation (87 genes) and lipid metabolism (25 genes) in L02 cells, and fibrosis (16 genes) in LX2 cells were reversed by Ad-DUSP22 (Supplementary Fig. 4r). This evidence concerns the gene DUSP22 and metabolic dysfunction-associated steatohepatitis.