Under NCD-feeding status beginning from week 0 to week 24, DUSP22HepKO did not develop any NASH-related hepatic phenotype when compared with DUSP22flox mice, as indicated by the similar hepatic histological structures, serum concentrations of ALT and AST, TNF-α, and IL-1β contents, and liver TG and TC levels (Supplementary Fig. 6g–j). This evidence concerns the gene TNF and metabolic dysfunction-associated steatohepatitis.