Next, hepatocyte-specific DUSP22 overexpression mice (DUSP22HepOE) were created using DUSP22 conditional knock in at the locus of Rosa26 in mice (DUSP22Rosa) based on Rosa26 conditional and/or inducible transgenesis to further confirm the regulatory function of DUSP22 in NASH. The gene discussed is DUSP22; the disease is metabolic dysfunction-associated steatohepatitis.