In the ex vivo transplanted mouse model, hepatocyte-specific DUSP22 overexpression (LV-DUSP22) transduction dramatically reduced liver weight, LW/BW ratio, blood glucose concentrations, fasting insulin levels, HOMA-IR values, and serum concentrations of ALT and AST in mice with a 24-week HFHC challenge; however, hepatocyte-specific DUSP22 knockdown (LV-shDUSP22) mice exhibited remarkably accelerated NASH phenotypes caused by HFHC, while no significant difference was observed in the body weight changes of all groups of mice (Fig. 7b–h). The gene discussed is DUSP22; the disease is metabolic dysfunction-associated steatohepatitis.