Western blot results showed that after inhibiting the PI3K/AKT signaling pathway, the enhanced expression of PLEKHH2 did not significantly promote the expression of proliferation- and invasion-related proteins (Fig. 4D, Supplementary Fig. 4A and B) and the proliferation and invasion abilities of lung cancer cells (Fig. 4E–G, Supplementary Fig. 4C–F). The gene discussed is PLEKHH2; the disease is lung cancer.