Next, to confirm if S-nitrosylation of CSF1R at Cys224, Cys278, and Cys830 is relevant for the effective reduction of tumor-promoting cytokines, we transfected 22RV1 cells with CSF1R plasmids (wild-type and three mutants) and exposed the cells to vehicle or CSF1R blockade (GW2580, 0.5 μM) for 48 hours. The gene discussed is CSF1R; the disease is neoplasm.