In summary, the findings of this study demonstrate that miR-133a-3p is involved in ovary IR in granulosa cells of obese PCOS ovaries via PI3K/AKT signaling. Thus, this study contributes to our understanding of the functional role and the underlying mechanism of miR-133a-3p in ovary IR, which have important reference value for the development of drugs for ovary insulin sensitization and the expansion of clinical obesity PCOS treatment. This evidence concerns the gene AKT1 and Obesity.