In the previous studies, the transcription of Toll-like receptor 3 (TLR3), retinoic acid-inducible gene I (RIG-I), and melanoma differentiation-associated gene 5 (MDA5), as well as several interferon-stimulated genes including OAS2, ISG15, and MX1, were strongly increased in human fibroblasts after ZIKV infection [16]. The gene discussed is ISG15; the disease is Zika virus infectious disease.