Propofol inhibited the cell viability, proliferation, migration, and invasion of lung cancer cells; all of which were associated with several pro-tumor genes (such as VEGFA, CTBP1 and CST7) were downregulated but several anti-tumor genes (such as NR4A3, RB1, and NME1) of lung cancer cells were upregulated after propofol treatment. This evidence concerns the gene RB1 and lung cancer.