To further confirm the role of NDUFA4 in the regulation of virus infection, we treated the low-permissive and permissive iPSC lines with mitochondrial complex IV inhibitor KCN (Hargreaves et al., 2007; Kilbride et al., 2011; Leavesley et al., 2008), and decreased percentages of ZIKV-E+ cells were seen in both permissive and low-permissive iPSC lines (ZIKVPR conditions: Figures 7C and 7D). This evidence concerns the gene COXFA4 and viral infectious disease.