In an ex-vivo approach for hemophilia B, a HC-Ad5 vectorcontaining an inducible gene-specific CRISPR/Cas9 system together with anadeno-associated virus containing the modified donor, and a HC-Ad5 vector withall the components were used to transduce liver cell lines stably expressingmutated canine factor IX gene (carrying a point mutation). The gene discussed is F9; the disease is hemophilia B.