To confirm that this placement of the GFP fusion has no decisive role in the markedly reduced interaction of the pathologic mutants with RGS19, we repeated the pull-downs with the C-terminally GFP-tagged Gαo variants, again revealing that the GNAO1 encephalopathy mutants lose the interactions with RGS19 (fig. The gene discussed is GNAO1; the disease is Encephalopathy.