PRNP and variant Creutzfeldt-Jakob disease: In order to assess the in vitro misfolding ability of non-glycosylated human PrP and how the lack of glycans could affect the human transmission barrier for BSE prions, TgNN6h mice brain homogenates were seeded in vitro with BSE isolates of different origins: classical cattle BSE (BSE), sheep (sBSE) and pig-passaged BSE (pBSE) [35] and human vCJD.