The analysis data showed that the DNA methylation levels of AACS (p=3.476E-08) and AASDH (p=2.293E-12) were lower in HCC tissues than in normal tissues, but ACSF2 (p=2.046E-04) was higher in HCC than in normal cases (Figure 7A–7D). The gene discussed is ACSF2; the disease is hepatocellular carcinoma.