CLDN1 and pachyonychia congenita: 2014). Thus, the function of SQS in the EMT of PC was examined. First, EMT was induced in 22Rv1 or PC-3 cells using TGF-β1 or TNF-α. Next, the impact of SQS on the EMT of PC cell lines was investigated. The results demonstrated that the mRNA and protein levels of EMT-related markers showed a significant change. After treatment with SQS, the expression of E-cadherin and claudin 1 was increased, while the expression of vimentin, N-cadherin, and α-smooth muscle actin was decreased in 22Rv1 or PC-3 cells (Figures 5 and 6).