Liver PI3K and Akt expression decreased, but NFkB expression increased after an LCA treatment in the H-L + HFD + INSR inhibitor group (Fig. 7H), indicating that LCA decreased the expression of INSR signaling downstream genes, resulting in the inhibition of INSR signaling and promoting the progression of liver dysfunction. The gene discussed is AKT1; the disease is Leber congenital amaurosis.