NFKB1 and Leber congenital amaurosis: Liver PI3K and Akt expression decreased, but NFkB expression increased after an LCA treatment in the H-L + HFD + INSR inhibitor group (Fig. 7H), indicating that LCA decreased the expression of INSR signaling downstream genes, resulting in the inhibition of INSR signaling and promoting the progression of liver dysfunction.