PIK3CA and cancer: Including all cancer-associated PI3K/RAS-mutations regardless of VAF (n = 69), 70% of the infants and all children carried at least 1 mutation (average infant: 2.2, range 0–13 and children: 5.1, range 1–10) and when several mutations were present, the VAFs were variable, consistent with the presence of multiple subclones (Figure 2A,E; Suppl.