The result indicated that CCL28, KLF2 and TNFSF18 did not significantly affect cell apoptosis (Figures 7A,C,E,F), while the knockdown of HMGB1 and TLR4 significantly increased the proportion of apoptotic of osteosarcoma cells (Figures 7B,D,F). The gene discussed is TNFSF18; the disease is osteosarcoma.