In this study, the impact of bone cell HIF signaling on antibacterial responses and pathologic changes in bone architecture was explored using genetic models with knockout of either Hif1a or a negative regulator of HIF-1α, Vhl. Deletion of Hif1a in osteoblast-lineage cells via Osx-Cre (Hif1aΔOB) had no impact on bacterial clearance or pathologic changes in bone architecture in a model of post-traumatic osteomyelitis. This evidence concerns the gene VHL and osteomyelitis.