Interestingly, TCA has been shown to increase FGF-21 levels in primary rat hepatocytes, perhaps showing a connection between bile acids and FGFs in the PCOS condition.29 Additionally, FGF-21, not FGF-19, was positively correlated with PCOS with and without obesity in our study. This evidence concerns the gene FGF19 and obesity due to melanocortin 4 receptor deficiency.