Evaluation of cell death in cancer cells is possible by colorimetric methods (MTT assay) and at the molecular level, which in the former is less sensitive and specific, but in the latter, includes the measurement of metastatic, angiogenic, and apoptotic factors such as Bcl-2, APAF1, ZEB1, VEGF, cyclin-D1, and caspase-3 by real-time PCR method, the more accurate test. This evidence concerns the gene APAF1 and cancer.