Here, the topical applications of EBN on AD mouse skin could significantly suppress: 1) the number of immune cells, e.g., Langerhans cell, basophil, eosinophil and mast cell; 2) the production of pro-inflammatory cytokines; 3) the serum level of IgE; and 4) the activation of canonical NF-κB signaling pathway. This evidence concerns the gene IGHE and Alzheimer disease.