Our results demonstrated that the treatment with 50% biogas slurry replacing for chemical N fertilizer significantly increased the relative abundances of genera Parasegetibacter, Gp5, and Povalibacter, while genera Steroidobacter, Latescibacteria_genera_incertae_sedis, Gp11, Gp15, Chondromyces, and Gp22 were positively correlated the biogas slurry addition in BS treatment. The gene discussed is S100A10; the disease is Bloom syndrome.