In summary, based on WGCNA and LASSO, combined with ssGSEA, six key genes (MVP, GRN, ERP29, RNF128, NFYB, and KPNA3) involved in dysferlinopathy have been identified, and their most important functions relate to protein degradation pathways, which in turn influence immune cell infiltration in dysferlinopathy. Here, NFYB is linked to neuromuscular disease caused by qualitative or quantitative defects of dysferlin.