Using WGCNA and LASSO algorithms and in vivo experiments, we have identified six key genes: MVP, GRN, ERP29, RNF128, NFYB and KPNA3. Significant abnormality in the expression levels of the six genes were revealed in dysferlinopathy, with MVP, GRN and ERP29 genes significantly upregulated, while RNF128, NFYB and KPNA3 significantly downregulated. The gene discussed is GRN; the disease is neuromuscular disease caused by qualitative or quantitative defects of dysferlin.