In Supplementary Figure S3A, 10 genes related to the prognosis of COAD patients were screened out from 68 differently-expressed genes related to copper metabolism by univariate regression analysis, among which 8 genes, amine oxidase, copper containing 3 (AOC3), CCND1, CD36, ceruloplasmin (CP), FABP4, glutathione S-transferase mu 1 (GSTM1), IGF1 and microtubule-associated protein tau (MAPT), were prognostic risk factors. The gene discussed is IGF1; the disease is colon adenocarcinoma.