HasterpKO mice showed normal morphology and growth (Extended Data Fig. 6a), yet male mice displayed glucose intolerance with insulin deficiency by 8 weeks, as well as fasting hyperglycaemia (glycaemia = 137 ± 16 mM in HasterpKO, 87 ± 5 mM in Hasterf/f littermates and 98 ± 4 mM in Pdx1-Cre; t-test P < 0.05) (Fig. 4a,b and Extended Data Fig. 6b). This evidence concerns the gene PDX1 and Hyperglycemia.