TNF and colonic neoplasm: In addition, previous research has also demonstrated that NF-κB activation is involved in IFN synthesis16, and alloferon-1 inhibited the degradation and phosphorylation of inhibitor of κB (IκB) induced by TNF-α in Colo205 colon cancer cells7, which provide clues to the basis of alloferon’s capability to stimulate IFN synthesis.