Mice in HF-SD group had higher 5hmC at Rac1 promoter compared to Diab group, and administration of SPT-siRNA ameliorated increase in 5hmC; 5hmC values in HF-SD/SPT-si group were significantly reduced, compared to HF-SD group (p < 0.05) (Fig. 7a). Here, AGXT is linked to hydrops fetalis.