These results indicate that nonLCR containing chimeric CPSF6/AD and CPSF6/NE proteins fail to engage with HIV-1 during infection, whereas LCR containing CPSF6/FU and CPSF6/CD can effectively substitute for WT CPSF6 during HIV-1 nuclear import and transport to NSs. The gene discussed is CPSF6; the disease is infection.