We observed upregulation of BST2, KLF2, and TUBA1A in ITP versus HC during the initial process of pseudotime, which participated in various aspects of cellular growth and development.28–30 In the middle of transition, many genes involved in immunomodulatory functions, such as CTSZ, FCER1G, IL3RA, IRF7, IGHM, and IGKC, were upregulated in ITP. Here, IGHM is linked to autoimmune thrombocytopenic purpura.