DEG analysis indicated that the top upregulated genes in MkP1 of ITP included HBB, HBG2, and AHSP, which were associated with erythropoiesis, while the top downregulated genes in MkP2 of ITP included CD9 and TUBB1, which are well-established genes related to thrombopoiesis, suggesting impaired megakaryopoiesis in ITP (Fig. 5a). This evidence concerns the gene HBB and autoimmune thrombocytopenic purpura.