In addition, the main signaling pathways were identified by KEGG enrichment analysis, it revealed the over-expressed genes were mainly involved in PI3K-Akt signaling pathway (hsa04151), Proteoglycans in cancer (hsa05205), Focal adhesion (hsa04510), Vascular smooth muscle contraction (hsa04270), Protein digestion and absorption (hsa04974), and Amoebiasis (hsa05146) (Figure 8 and Table S7a-b). This evidence concerns the gene AKT1 and cancer.