As shown in Figure 5(a), nine RNFs (RNF220, RNF25, TRIM25, RNF216P1, RNF115, TRAIP, RNF2, RNF157, and RNF145) were found to be upregulated in HCC patients with advanced grade (p < 0.05). This evidence concerns the gene RNF2 and hepatocellular carcinoma.