The Kaplan−Meier curve analysis (log-rank test) results showed that the overall survival rate of osteosarcoma patients with SPAG6 positive expression was significantly less than that with SPAG6 negative expression (Figure 3A), while the rate was adverse in NM23 expression (Figure 3B) with p < 0.05 for all. This evidence concerns the gene SPAG6 and osteosarcoma.